Crew 315 End-of-Mission Research Report 02May2025

[title End-Mission Research Report – May 2nd]
[category science-report]

End-of-Mission Research Report – Crew 315

Summary of Crew Research Projects:

Title: Methodology Extending Mobility Range on Mars

Principal Investigator: David Laude
Description: Mobility on Mars is key to any mission for maximizing scientific gains. Main mobility for humans is motorized rovers with limited range. Mobility can be extended for examination of more remote objects. Objects of interest can be observed from rover accessible vantage points. Two observations can be used to triangulate object position (no GPS on Mars). Position can be found or placed on map to determine travel range. If range is beyond rover range, but within rover + foot + drone range then range can be extended by foot and then deploying an FPV drone/helicopter. Drone can collect close up HD photos.
Objective: An EVA team will set out on EVA with a small drone equipped with HD camera and FPV capability. EVA team will follow a planned course from maps. When rover is at maximum range (real or simulated), EVA crew will set out on foot with drone. Once EVA crew is close enough to the object, the drone pilot will launch it. Drone pilot will fly drone in full sim suit while drone spotter(s) stand nearby. Drone will acquire the needed object images from close up Image data will be retrieved from drone in Hab for analysis to determine if mission was a success. Project methods will be reviewed for success or needed improvements
Research Summary: The project has completed with a close encounter with the Monolith objective by drone after having triangulated its position from two vantage points and placing object on map. From that we plotted a course by rover as close as we could get followed by a short hike up a hill where the drone was launched. This shows the usefulness of the methodology for examination of remote objects further than one would ordinarily expect.

Title: Evaluating Drone Piloting During EVA on Mars
Principal Investigator: David Laude
Description: With the success of Ingenuity paving the way, piloted drones will undoubtedly be used by humans on Mars. The purpose of this project is to study drone piloting with EVA suit and to evaluate any operational impediments. Co-investigators will evaluate drone flight control performance on standardized flight patterns, making use of URC fields and possibly other locations. Co-Investigators will rate each flight through several metrics. No EVA suit flights will take place prior to and/or just after sim.
Objectives: Metrics like accuracy (measured distance to center of target) and speed (time) of flying drone to marked targets of varying ranges will be evaluated via comparative analysis. Comments on difficulties experienced will also be documented.
Research Summary: This project is completed. It has shown what one would expect for piloting a drone in EVA suit. Poorer visibility in EVA can cause temporary loss of drone sighting by both naked eye and FPV display. Displays need to be brighter. In addition, the wearing of gloves impedes fine drone control.

Title: Illustrating a Mars Analog Mission as an artist.
Principal Investigator: Timothy Gagnon
Description:In March 1962, NASA Administrator James Webb addressed a two-paragraph memorandum to NASA Public Affairs Director Hiden T. Cox about the possibility of bringing in artists to highlight the agency’s achievements in a new way. In it, he wrote, “We should consider in a deliberate way just what NASA should do in the field of fine arts to commemorate the … historic events” of America’s initial steps into space.
Shortly thereafter, NASA employee and artist James Dean was tasked with implementing NASA’s brand-new art program. Working alongside National Art Gallery Curator of Painting H. Lester Cooke, he created a framework to give artists unparalleled access to NASA missions at every step along the way, such as suit-up, launch and landing activities, and meetings with scientists and astronauts. Over the years, NASA artwork has helped spark national pride and accomplishment. Technology, whether from the 1960s or today, documented these missions extensively, but artists are able to pull in emotion and imagination unlike data-collecting machinery. The relationship between science and art continues to inspire the public and inform us of current missions. When I was invited to participate in a MDRS analog mission as an artist, I immediately thought of contributing the same way as the artists involved in the NASA Art Program of the 1960’s and 1970’s.
Objectives: To document my experience and that of my crew mates by creating digital and fine art of our increment. I have already designed our mission patch, our crew portraits and a "Space Flight Awareness" themed crew poster. I intend to bring my iPhone camera, possibly my iPad as well as a sketch pad along with pens and colored pencils to sketch while there and then turning those into finished art post mission.
Research Summary: Due to the limited field of view offered by the suit helmet and the limited dexterity of the gloves, sketching during an EVA proved impossible. However, I was able to take and request certain photos inspired by the Apollo lunar missions and paintings by artists I admire to create tributes to those missions and those artists. This was accomplished during four EVAs of mine and multiple EVAs by my crew mates. Together we have assembled a portfolio of photographs that will be the basis of a series of art pieces based on the theme, “What it looked like vs What it felt like.” Analog vs Artemis missions to Mars. I will donate those pieces to The Mars Society to hopefully use in their fund raising efforts.

Title: Essay for Harper’s Magazine

Principal investigator: Elena Saavedra Buckley

Description: The primary reason for my visit to the MDRS is to write an immersive, in-depth reported essay for Harper’s Magazine, to run as a feature at some point later in the year. This piece is assigned at Harper’s, where I am an editor, and has been approved by the MDRS via Michael Stoltz, the media and PR liaison.

Objectives: The aim of the article is not only to capture the experience of our mission, but to zoom out and consider the purpose of Martian simulations, of eventual Mars missions, and the place these phenomena have in the American imagination today.

Research Summary: My reporting went well, and I was able to talk individually with my crewmates and with everyone as a group multiple times. I’m excited to bring all my reporting to Earth, continue my research, and put it all together in 1g.

Title: Examining oyster mushroom growth in a Martian greenhouse environment

Principle investigator: Elena Saavedra Buckley

Description: Mushrooms are an easy to grow, nutritious source of food that can be transported in remarkably compact ways. (Beyond culinary uses, fungi structures are strong and lightweight, and NASA has studied the feasibility of using them for Martian architecture, or “mycotecture.”)

Objectives: Use a pre-made grow kit to grow oyster mushrooms in the Greenhab to gain information on possible hiccups and problems with mushroom growing in a sealed, arid environment; and, ideally, eat them.

Research Summary: Sadly my mushroom kit has seemingly failed. I sprayed it regularly and installed a humidity tent, and I followed all kit directions, but the “pins” never formed. Technically they could form in the next few days, but it’s more likely that the conditions were too hot or dry for blue oysters. Green mold did start forming on the exposed spores, so I imagine that indicates some kind of decay.

Title: Measuring soil desiccation patterns near the MDRS

Principle investigator: Elena Saavedra Buckley

Description: Desiccation cracks in soil form as moisture evaporates, leaving behind polygonal patterns that have been observed in terrestrial desert environments. On Mars, these features provide insight into past hydrological conditions, soil composition, and potential habitability. By studying desiccation patterns in the Mars-like environment of the MDRS, I will better understand how similar features on Mars might have formed, and learn more about how soil evaporation occurs.

Objectives: Measure various soil desiccation pattern areas and, in the science dome, do a simple experiment on soil samples to see how long cracks take to form.

Research Summary: I collected five diverse soil samples from around the MDRS—ranging from gravel to clay—and measured variables regarding their desiccation powers in the field. In the Science Dome, I mixed consistent amounts of soil and water and packed them into petri dishes, where I placed them in the GreenHab (in order to get accurate temperature and humidity readings); the majority desiccated over the course of two days, with two samples not yet desiccating, suggesting that their desiccation patterns in the field required either drier conditions or more surface tension. I will write up the comparisons between the spread of measurements in the field and in the patterns in the lab and further analyze how the soils’ conditions related to their desiccation speeds.

Title: EVA Connectivity Kit
Principal Investigator: Michael Andrews
Description: By combining commercial off-the-shelf products, I developed a portable kit that can be taken on EVAs to provide internet connectivity to crew members. This has various benefits: sending data back to the station, enhanced communications, and en-situ research while on EVA. Objectives: Over the course of 3 EVAs, confirm efficacy of kit and measure its performance parameters: battery life, upload speed, download speed, weight.
Research Summary: I have been able to demonstrate that a Starlink mini and 20,000mAh battery pack can be easily carried and deployed on an analog EVA. Over the course of 5 tests and 3 EVAs, an average expected life of 171 minutes, download speed of 140 Mbps, and upload speed of 14.5 Mbps was observed. I was able to regularly bring this kit on future EVAs to support the crew and my 3D scanning project’s objectives.

Title: 3D Mapping of Samples
Principal Investigator: Michael Andrews
Description: To prevent physical extraction of geological samples on EVAs, I demonstrated 3D mapping technology as a way to create "digital twins" of specimens. This will also include engineering hardware on station.
Objectives: Determine how quickly samples can be recorded in station and on EVA, including sending them to the station via the Connectivity Kit above.
Research Summary: Over the course of six EVAs, I was able to collect samples to return and scan in the Science Dome and scan samples en-situ using my equipment. I scanned a total of 14 samples, 3 of which were en-situ (see Figure 1). The samples were a variety of colors and textures, and ranging in weights up to 610g and lengths of 6.25”. The activity would first take me up to 2 hours per sample, but I have determined a technique (one geometry scan and two texture scans) to construct the EVA shroud in 9 minutes and perform all scanning operations in 35 minutes. The output file (.obj file type) can quickly be shared to a Google Drive via Starlink and be viewed by other crew members in the station while the EVA is ongoing.

Title: 100cameras Method: Photography as a Tool to Mitigate Psychological Stress in Space

Principal Investigator: Urban Koi, HSO

Project Description: Space exploration presents unique psychological challenges for astronauts, particularly during long-duration missions where isolation, confinement, and distance from Earth can lead to significant emotional and mental stress. As humanity advances toward becoming a multi-planetary species, addressing these psychological effects is crucial for the success of future missions to the Moon, Mars, and beyond. Developed over 15 years of research and practice, the 100cameras Method leverages photography as a dynamic tool for self-expression, fostering emotional intelligence, resilience, and community-building skills. The 100cameras Method has been recognized by the United Nations University Centre for Policy Research (UNU-CPR), UNIDIR, and UNICEF for its positive impact on empowerment globally. By integrating the 100cameras Method into the daily lives of analog astronauts, we aim to provide future astronauts with a structured yet flexible approach to document their experiences, process emotions, and strengthen connections with their environment and peers, combating the psychological effects of space travel.

Objectives: (1) To evaluate the effectiveness of the 100cameras Method in enhancing emotional intelligence and resilience among analog astronauts. (2) To assess the impact of photography-based self-expression on the well-being of individuals in isolated or extreme environments, such as analog and space missions. (3) To analyze the potential of the 100cameras Method as a scalable intervention for various populations facing psychological challenges. (4) To integrate the 100cameras Method into future astronaut psychological wellness toolkits.

Project Completion: Crew Phoenix (MDRS-315) has successfully completed 8 of 8 modules. All MDRS-315 Analog Astronauts are now 100cameras Graduates and have the 100cameras Method in their Psychological Wellness Toolkit for future missions in Isolated, Confined, and Extreme Environments. Congratulations to Crew Phoenix!

(1) Introduction: 100cameras Overview + Pre-Course Survey.

(2) Composition & Storytelling: Composition Techniques—Telling A Story, Leading Lines & Vanishing Point, Repetition & Patterns, Symmetry, Point Of View, Rule Of Thirds. Each crew member captured 10+ images pertaining to the module exercise.

(3) Camera Tool-Belt: This module focused on teaching analog astronauts how to capture the moment and the story as they see it best. Through learning the camera equipment and its functions, analog astronauts learn techniques such as exposure, aperture, and flash. Each crew member captured 10+ images pertaining to the module exercise.

(4) Range of Feelings: The activities showed that stories can be told in a more compelling and engaging way when practicing the different composition techniques and when utilizing the tool-belt techniques to adjust the camera to work best within the environment at hand. This module focused on how different feelings and emotions can be expressed through photography, enabling a fuller narrative to be communicated and experienced through images. Each crew member captured 10+ images pertaining to the module exercise.

(5) Something of Me: This module focused on exploring how a portion of a person’s individual stories, such as their interests, experiences, circumstances, and ideas can impact how someone sees themselves. It’s a guide to explore some of the pieces that make each person who they are as individuals and relate to how stories are shaped by these elements. Looking inward and spending time with oneself can influence how a person sees their own story and perspective—and how they tell it and share it. Each crew member captured 10+ images pertaining to the module exercise. Each crew member captured 10+ images pertaining to the module exercise.

(6) Map My Story: Through the "Map My Story" exercise, crew members considered the past, present, and where the future might take them by illustrating a life map. They reflected on who they are today because of their past experiences and how these experiences have helped to shape them. Crew members were encouraged to envision and dream about their future and who they want to become.

(7) Portraiture: This module focused on how all of the tools that have been learned thus far can contribute to creating photos that reflect the journey as well as the inner self through portraits and self-portraits. Crew members participated in activities that help explore different ways to portray themselves and others through photography, both in direct and abstract, creative ways. Each crew member captured 10+ images pertaining to the module exercise.

(8) Your Role in the World: This module focused on how to tie together multiple photographs to tell one cohesive story. Crew members created a “portfolio” or group of images which relate to one another, rather than one single image by itself. Crew members engaged in activities that help practice creating a “Central Theme” portfolio.

(9) Graduation: EachMDRS-315 crew member graduated and received a 100cameras Certificate of Completion.

Crew 315 End-of-Mission Research Report 02May2025

On Fri, May 2, 2025 at 9:21 PM David Steinhour <dsteinhour> wrote:

[title End-Mission Research Report – May 2nd]
[category science-report]

End-of-Mission Research Report – Crew 315

Summary of Crew Research Projects:

Title: Methodology Extending Mobility Range on Mars

Principal Investigator: David Laude
Description: Mobility on Mars is key to any mission for maximizing scientific gains. Main mobility for humans is motorized rovers with limited range. Mobility can be extended for examination of more remote objects. Objects of interest can be observed from rover accessible vantage points. Two observations can be used to triangulate object position (no GPS on Mars). Position can be found or placed on map to determine travel range. If range is beyond rover range, but within rover + foot + drone range then range can be extended by foot and then deploying an FPV drone/helicopter. Drone can collect close up HD photos.
Objective: An EVA team will set out on EVA with a small drone equipped with HD camera and FPV capability. EVA team will follow a planned course from maps. When rover is at maximum range (real or simulated), EVA crew will set out on foot with drone. Once EVA crew is close enough to the object, the drone pilot will launch it. Drone pilot will fly drone in full sim suit while drone spotter(s) stand nearby. Drone will acquire the needed object images from close up Image data will be retrieved from drone in Hab for analysis to determine if mission was a success. Project methods will be reviewed for success or needed improvements
Research Summary: The project has completed with a close encounter with the Monolith objective by drone after having triangulated its position from two vantage points and placing object on map. From that we plotted a course by rover as close as we could get followed by a short hike up a hill where the drone was launched. This shows the usefulness of the methodology for examination of remote objects further than one would ordinarily expect.

Title: Evaluating Drone Piloting During EVA on Mars
Principal Investigator: David Laude
Description: With the success of Ingenuity paving the way, piloted drones will undoubtedly be used by humans on Mars. The purpose of this project is to study drone piloting with EVA suit and to evaluate any operational impediments. Co-investigators will evaluate drone flight control performance on standardized flight patterns, making use of URC fields and possibly other locations. Co-Investigators will rate each flight through several metrics. No EVA suit flights will take place prior to and/or just after sim.
Objectives: Metrics like accuracy (measured distance to center of target) and speed (time) of flying drone to marked targets of varying ranges will be evaluated via comparative analysis. Comments on difficulties experienced will also be documented.
Research Summary: This project is completed. It has shown what one would expect for piloting a drone in EVA suit. Poorer visibility in EVA can cause temporary loss of drone sighting by both naked eye and FPV display. Displays need to be brighter. In addition, the wearing of gloves impedes fine drone control.

Title: Illustrating a Mars Analog Mission as an artist.
Principal Investigator: Timothy Gagnon
Description:In March 1962, NASA Administrator James Webb addressed a two-paragraph memorandum to NASA Public Affairs Director Hiden T. Cox about the possibility of bringing in artists to highlight the agency’s achievements in a new way. In it, he wrote, “We should consider in a deliberate way just what NASA should do in the field of fine arts to commemorate the … historic events” of America’s initial steps into space.
Shortly thereafter, NASA employee and artist James Dean was tasked with implementing NASA’s brand-new art program. Working alongside National Art Gallery Curator of Painting H. Lester Cooke, he created a framework to give artists unparalleled access to NASA missions at every step along the way, such as suit-up, launch and landing activities, and meetings with scientists and astronauts. Over the years, NASA artwork has helped spark national pride and accomplishment. Technology, whether from the 1960s or today, documented these missions extensively, but artists are able to pull in emotion and imagination unlike data-collecting machinery. The relationship between science and art continues to inspire the public and inform us of current missions. When I was invited to participate in a MDRS analog mission as an artist, I immediately thought of contributing the same way as the artists involved in the NASA Art Program of the 1960’s and 1970’s.
Objectives: To document my experience and that of my crew mates by creating digital and fine art of our increment. I have already designed our mission patch, our crew portraits and a "Space Flight Awareness" themed crew poster. I intend to bring my iPhone camera, possibly my iPad as well as a sketch pad along with pens and colored pencils to sketch while there and then turning those into finished art post mission.
Research Summary: Due to the limited field of view offered by the suit helmet and the limited dexterity of the gloves, sketching during an EVA proved impossible. However, I was able to take and request certain photos inspired by the Apollo lunar missions and paintings by artists I admire to create tributes to those missions and those artists. This was accomplished during four EVAs of mine and multiple EVAs by my crew mates. Together we have assembled a portfolio of photographs that will be the basis of a series of art pieces based on the theme, “What it looked like vs What it felt like.” Analog vs Artemis missions to Mars. I will donate those pieces to The Mars Society to hopefully use in their fund raising efforts.

Title: Essay for Harper’s Magazine

Principal investigator: Elena Saavedra Buckley

Description: The primary reason for my visit to the MDRS is to write an immersive, in-depth reported essay for Harper’s Magazine, to run as a feature at some point later in the year. This piece is assigned at Harper’s, where I am an editor, and has been approved by the MDRS via Michael Stoltz, the media and PR liaison.

Objectives: The aim of the article is not only to capture the experience of our mission, but to zoom out and consider the purpose of Martian simulations, of eventual Mars missions, and the place these phenomena have in the American imagination today.

Research Summary: My reporting went well, and I was able to talk individually with my crewmates and with everyone as a group multiple times. I’m excited to bring all my reporting to Earth, continue my research, and put it all together in 1g.

Title: Examining oyster mushroom growth in a Martian greenhouse environment

Principle investigator: Elena Saavedra Buckley

Description: Mushrooms are an easy to grow, nutritious source of food that can be transported in remarkably compact ways. (Beyond culinary uses, fungi structures are strong and lightweight, and NASA has studied the feasibility of using them for Martian architecture, or “mycotecture.”)

Objectives: Use a pre-made grow kit to grow oyster mushrooms in the Greenhab to gain information on possible hiccups and problems with mushroom growing in a sealed, arid environment; and, ideally, eat them.

Research Summary: Sadly my mushroom kit has seemingly failed. I sprayed it regularly and installed a humidity tent, and I followed all kit directions, but the “pins” never formed. Technically they could form in the next few days, but it’s more likely that the conditions were too hot or dry for blue oysters. Green mold did start forming on the exposed spores, so I imagine that indicates some kind of decay.

Title: Measuring soil desiccation patterns near the MDRS

Principle investigator: Elena Saavedra Buckley

Description: Desiccation cracks in soil form as moisture evaporates, leaving behind polygonal patterns that have been observed in terrestrial desert environments. On Mars, these features provide insight into past hydrological conditions, soil composition, and potential habitability. By studying desiccation patterns in the Mars-like environment of the MDRS, I will better understand how similar features on Mars might have formed, and learn more about how soil evaporation occurs.

Objectives: Measure various soil desiccation pattern areas and, in the science dome, do a simple experiment on soil samples to see how long cracks take to form.

Research Summary: I collected five diverse soil samples from around the MDRS—ranging from gravel to clay—and measured variables regarding their desiccation powers in the field. In the Science Dome, I mixed consistent amounts of soil and water and packed them into petri dishes, where I placed them in the GreenHab (in order to get accurate temperature and humidity readings); the majority desiccated over the course of two days, with two samples not yet desiccating, suggesting that their desiccation patterns in the field required either drier conditions or more surface tension. I will write up the comparisons between the spread of measurements in the field and in the patterns in the lab and further analyze how the soils’ conditions related to their desiccation speeds.

Title: EVA Connectivity Kit
Principal Investigator: Michael Andrews
Description: By combining commercial off-the-shelf products, I developed a portable kit that can be taken on EVAs to provide internet connectivity to crew members. This has various benefits: sending data back to the station, enhanced communications, and en-situ research while on EVA. Objectives: Over the course of 3 EVAs, confirm efficacy of kit and measure its performance parameters: battery life, upload speed, download speed, weight.
Research Summary: I have been able to demonstrate that a Starlink mini and 20,000mAh battery pack can be easily carried and deployed on an analog EVA. Over the course of 5 tests and 3 EVAs, an average expected life of 171 minutes, download speed of 140 Mbps, and upload speed of 14.5 Mbps was observed. I was able to regularly bring this kit on future EVAs to support the crew and my 3D scanning project’s objectives.

Title: 3D Mapping of Samples
Principal Investigator: Michael Andrews
Description: To prevent physical extraction of geological samples on EVAs, I demonstrated 3D mapping technology as a way to create "digital twins" of specimens. This will also include engineering hardware on station.
Objectives: Determine how quickly samples can be recorded in station and on EVA, including sending them to the station via the Connectivity Kit above.
Research Summary: Over the course of six EVAs, I was able to collect samples to return and scan in the Science Dome and scan samples en-situ using my equipment. I scanned a total of 14 samples, 3 of which were en-situ (see Figure 1). The samples were a variety of colors and textures, and ranging in weights up to 610g and lengths of 6.25”. The activity would first take me up to 2 hours per sample, but I have determined a technique (one geometry scan and two texture scans) to construct the EVA shroud in 9 minutes and perform all scanning operations in 35 minutes. The output file (.obj file type) can quickly be shared to a Google Drive via Starlink and be viewed by other crew members in the station while the EVA is ongoing.

Title: 100cameras Method: Photography as a Tool to Mitigate Psychological Stress in Space

Principal Investigator: Urban Koi, HSO

Project Description: Space exploration presents unique psychological challenges for astronauts, particularly during long-duration missions where isolation, confinement, and distance from Earth can lead to significant emotional and mental stress. As humanity advances toward becoming a multi-planetary species, addressing these psychological effects is crucial for the success of future missions to the Moon, Mars, and beyond. Developed over 15 years of research and practice, the 100cameras Method leverages photography as a dynamic tool for self-expression, fostering emotional intelligence, resilience, and community-building skills. The 100cameras Method has been recognized by the United Nations University Centre for Policy Research (UNU-CPR), UNIDIR, and UNICEF for its positive impact on empowerment globally. By integrating the 100cameras Method into the daily lives of analog astronauts, we aim to provide future astronauts with a structured yet flexible approach to document their experiences, process emotions, and strengthen connections with their environment and peers, combating the psychological effects of space travel.

Objectives: (1) To evaluate the effectiveness of the 100cameras Method in enhancing emotional intelligence and resilience among analog astronauts. (2) To assess the impact of photography-based self-expression on the well-being of individuals in isolated or extreme environments, such as analog and space missions. (3) To analyze the potential of the 100cameras Method as a scalable intervention for various populations facing psychological challenges. (4) To integrate the 100cameras Method into future astronaut psychological wellness toolkits.

Project Completion: Crew Phoenix (MDRS-315) has successfully completed 8 of 8 modules. All MDRS-315 Analog Astronauts are now 100cameras Graduates and have the 100cameras Method in their Psychological Wellness Toolkit for future missions in Isolated, Confined, and Extreme Environments. Congratulations to Crew Phoenix!

(1) Introduction: 100cameras Overview + Pre-Course Survey.

(2) Composition & Storytelling: Composition Techniques—Telling A Story, Leading Lines & Vanishing Point, Repetition & Patterns, Symmetry, Point Of View, Rule Of Thirds. Each crew member captured 10+ images pertaining to the module exercise.

(3) Camera Tool-Belt: This module focused on teaching analog astronauts how to capture the moment and the story as they see it best. Through learning the camera equipment and its functions, analog astronauts learn techniques such as exposure, aperture, and flash. Each crew member captured 10+ images pertaining to the module exercise.

(4) Range of Feelings: The activities showed that stories can be told in a more compelling and engaging way when practicing the different composition techniques and when utilizing the tool-belt techniques to adjust the camera to work best within the environment at hand. This module focused on how different feelings and emotions can be expressed through photography, enabling a fuller narrative to be communicated and experienced through images. Each crew member captured 10+ images pertaining to the module exercise.

(5) Something of Me: This module focused on exploring how a portion of a person’s individual stories, such as their interests, experiences, circumstances, and ideas can impact how someone sees themselves. It’s a guide to explore some of the pieces that make each person who they are as individuals and relate to how stories are shaped by these elements. Looking inward and spending time with oneself can influence how a person sees their own story and perspective—and how they tell it and share it. Each crew member captured 10+ images pertaining to the module exercise. Each crew member captured 10+ images pertaining to the module exercise.

(6) Map My Story: Through the "Map My Story" exercise, crew members considered the past, present, and where the future might take them by illustrating a life map. They reflected on who they are today because of their past experiences and how these experiences have helped to shape them. Crew members were encouraged to envision and dream about their future and who they want to become.

(7) Portraiture: This module focused on how all of the tools that have been learned thus far can contribute to creating photos that reflect the journey as well as the inner self through portraits and self-portraits. Crew members participated in activities that help explore different ways to portray themselves and others through photography, both in direct and abstract, creative ways. Each crew member captured 10+ images pertaining to the module exercise.

(8) Your Role in the World: This module focused on how to tie together multiple photographs to tell one cohesive story. Crew members created a “portfolio” or group of images which relate to one another, rather than one single image by itself. Crew members engaged in activities that help practice creating a “Central Theme” portfolio.

(9) Graduation: EachMDRS-315 crew member graduated and received a 100cameras Certificate of Completion.

Research Report -April 29th

[category science-report]

Title: Methodology Extending Mobility Range on Mars

Principal Investigator: David Laude
Current Progress: The project has completed with a close encounter with the Monolith objective by drone after having triangulated its position from two vantage points and placing object on map. From that we plotted a course by rover as close as we could get followed by a short hike up a hill where the drone was launched. This shows the usefulness of the methodology for examination of remote objects further than one would ordinarily expect.

Title: Evaluating Drone Piloting During EVA on Mars
Principal Investigator: David Laude
Current Progress: This project is completed. It has shown what one would expect for piloting a drone in EVA suit. Poorer visibility in EVA can cause temporary loss of drone sighting by both naked eye and FPV display. Displays need to be brighter. In addition, the wearing of gloves impedes fine drone control.

Title: EVA Connectivity Kit

Principal Investigator: Michael Andrews – Crew Engineer

Current Progress: This project is complete! I have been able to demonstrate that a Starlink mini and 20,000mAh battery pack can be easily carried and deployed on an analog EVA. Here are the series of tests I performed over the last few sols:

· On 4/21, I tested the kit before simulation started at the Observatory. I was able to get all crew members to connect to it for use on EVAs. It took a bit longer to connect for the first time in Utah (~5 minutes), and it used 16% of the battery pack within 30 minutes. Download speeds peaked at 86 Mbps and upload speeds peaked at 19.0 Mbps.

· On 4/22, I tested the Starlink inside the Science Dome to see how it would consume battery when unable to connect. I turned it on at 1533 and by 1616, the battery pack had reduced from 84% to 58%. No speed test was conducted.

· On 4/23, I took the kit on its first EVA. The kit was deployed and powered up at 1435, it connected at 1438, and I shut down the unit at 1519. During this time, 27% of the battery pack was consumed. Download speeds peaked at 107 Mbps and upload speeds peaked at 10.4 Mbps.

· On 4/24, I took the kit on a longer EVA. The kit was deployed and powered up at 1451, it connected at 1453, and I shut down the unit at 1551. During this time, 35% of the battery pack was consumed. Download speeds peaked at 129 Mbps and upload speeds peaked at 14.1 Mbps.

Under these four tests, an average expected life of 171 minutes, download speed of 140 Mbps, and upload speed of 14.5 Mbps was observed. I plan to regularly bring this kit on future EVAs to support the crew and my 3D scanning project’s future objectives.

3D Scanning of Samples: This project is on track to complete by the end of our mission. I have been able to collect various samples of shapes, textures, and colors on EVAs. I have learned the software that came along with my Seal scanner known as JMStudio to create 3D files (.obj, .ply, and .stl types) of each sample before returning it to the collection site.

Title: Measuring soil desiccation patterns near the MDRS
Principle investigator: Elena Saavedra Buckley

Current Progress: I’ve collected five diverse soil samples from areas around the MDRS—near Candor Chasma, the turnoff to Galileo Road, the road of Somerville Overlook, off Galileo near Compass Rock, and off Cow Dung near the turnoff to the Sea of Shells. In the field, I took measurements of the soil desiccation sections and their depth within a square foot of the desiccated soil, which is smaller than my proposal’s initial area, since a meter ended up feeling larger than needed to measure. Tomorrow (sol 7), I’ll prepare the soils in my petri dishes and spend the rest of my mission watching and documenting their desiccation patterns as they dry in the GreenHab.

Title: Examining oyster mushroom growth in a Martian greenhouse environment

Principle investigator: Elena Saavedra Buckley

Current Progress: I opened and started my mushroom kit, along with building a humidity tent, in the GreenHab on Sol 0. I’ve been misting its opening at least twice a day, and no mushroom “pins” have formed. While the pins can form at any point between 5 and 10 days, I’m worried they might not appear because the GreenHab has been so hot on occasion, sometimes getting to 106 degrees. We’ll see if it makes any progress.

Title: Essay for Harper’s Magazine

Principal Investigator: Elena Saavedra Buckley

Current Progress: My reporting is going well! Since it encompasses my entire experience at the MDRS, I don’t have any particular updates beyond being grateful to my crewmates for being up for interviews, observation, and discussion.

Title: 100cameras Method: Photography as a Tool to Mitigate Psychological Stress in Space

Principal Investigator:Urban Koi, HSO

Project Progress:MDRS-315 has completed 5 of 8 modules.

Title: Illustrating a Mars Analog Mission as an artist.

Principal Investigator: Tim Gagnon

Project Progress: My goal when assigned was to artistically represent our MDRS mission as an embedded artist.

As of today as we complete week one, I have taken inspiration from the Apollo program to replicate some of the most notable paintings by Alan Bean, Ed Hengevelde and Chris Calle. It was hoped that while on an EVA I would be able to create some rough sketches. Due to the limited visibility of the helmet and limited dexterity of the gloves, that proved impossible. Post mission, I’ll draw inspiration from our crew photo album to create a series of art pieces illustrating MDRS-315.

Astronomy Report – April 11th

[category 

astronomy-report]

Crew 314 Astronomy Report 11Apr2025

Name: Louis Baltus

Crew: 314

Date: 11Apr2025

MDRS ROBOTIC OBSERVATORY

Objects to be Imaged this Evening: /

Images submitted with this report: /

Problems Encountered: /

MUSK OBSERVATORY

Solar Features Observed: Full solar disk observed and imaged today as first observation. During the next day, I’ll try to zoom in on the details I will see.

Images submitted with this report (2) :The one of the chromosphere and the one with the prominences (one of them is jpg)

Problems Encountered:

-At first, I couldn’t see anything so I sent an email to Peter Detterline. Thanks to his advice I succeeded and observed the sun for the first time. What a wonderful feeling !

-Couldn’t superpose the two images properly, the one with the prominences keep coming as first layer. As it’s the first time for me using all that software, I’ll keep trying and learning and see if I manage to get the expected result.

Mid-mission Research Report – April 12th

[category science-report]

Mid mission report – Crew 314

Crew 314 – First Days on Mars: Adapting, Exploring, and Connecting

Crew 314 officially landed on the surface of Mars on April 6, 2025, at noon Earth time. Upon arrival, we quickly familiarize ourselves with the station and, after a restorative night’s sleep, began working on our respective experiments and preparing for the first EVAs.

The first two sols were particularly intense, filled with a fast-paced sequence of reports, extravehicular activities, experiment setup, station tasks, and the necessary adjustments to the Martian simulation lifestyle. A bit of confusion surrounding reporting procedures during these initial days added to the challenge, but the crew quickly adapted and found its rhythm.

Over the following three sols, although the schedule remained full, we were able to manage our tasks more efficiently. This allowed us to take moments to truly appreciate the experience of living and working on “Mars”: admiring the stunning landscape, sharing moments of team bonding through cooking, card games, and informal discussions.

These early days have laid the groundwork for a cohesive, resilient, and motivated crew, ready to make the most of the mission ahead.

Experiments:

This section provides an overview of the current status and recent developments in the various research projects being conducted by the crew. Each experiment continues to evolve in alignment with its objectives.

Odile Hilgers (Health and Safety Officer):

As Health and Safety Officer for this analog Martian mission, I am leading a series of six medical simulations designed to assess crisis management and team coordination in an isolated and confined environment. These scenarios are inspired by realistic medical emergencies and are adapted to the operational constraints of life in a Martian habitat. The program is structured to gradually increase in complexity and immersion: the first two simulations serve as training exercises, the next two are designed as Earth-based medical scenarios, and the final two will simulate emergencies occurring on Mars. Each simulation unfolds in three phases: a briefing, during which participants receive clinical background on the patient, including medical history and the story of the present illness; the simulation itself, involving three role-players and three observers; and finally, a 30 to 45-minute debriefing session centered around Crisis Resource Management (CRM) principles. During the debrief, all participants complete the Ottawa Global Rating Scale (Ottawa GRS), providing a structured evaluation of team performance. So far, three scenarios have been conducted, all taking place inside the Hab, both on the upper and lower decks. Data analysis will be conducted once all six simulations are completed, in order to evaluate behavioral patterns, decision-making processes, and overall team efficiency in high-stress situations.

Bérengère Bastogne (GreenHab Officer):

The experiments conducted at the Mars Desert Research Station are part of my doctoral thesis. The main objective is to evaluate the impact of Martian environmental stresses – UV radiation (A, B and C), temperature (hot-cold cycles), gravity and substrate (regolith) – on arbuscular mycorrhizal fungi (AMF). These fungi are obligate symbionts that associate with plant roots and can supply them up to 80% of total phosphorus and nitrogen. As one of the most important mutualistic microorganisms for global food production, AMF are essential elements to be considered for the development of future colonies on Mars.

Understanding how AMF respond to environmental conditions is critical. Since they are closely associated with plants, any parameter affecting spores could impact essential AMF functions and indirectly impact plant growth and plant health. However, despite their crucial role, little is known about how these environmental stresses affect these microorganisms. Therefore, expanding our knowledge in this area is crucial.

My research is divided into two experiments. The first aims to study the effects of these stresses on spore germination. The second focuses on the ability of spores, after exposure to stresses, to associate with plant roots.

The initial step involved estimating the number of spores in 10 g soil (to prepare for the first and second experiments). I then exposed the soil – containing spores – in Petri dishes or Falcon tubes to different conditions for 48h. For the germination study, approximately 10 g of soil (containing 30-40 spores) was used per condition. For the root association study, I used 10 g per condition, with six replicates.

To test the substrate stress, I isolated spores from 6x10g of soil and transferred them into regolith. The environmental conditions were applied as follows:

Temperature: Petri dishes were placed outside (near the entrance of the ScienceDome)

Gravity: Falcon tubes were attached to the Random Positioning Machine (RPM) – placed in the ScienceDome at room temperature

UV: Petri dishes were placed under a UV lamp in the ScienceDome at room temperature

Substrate: Spores were placed in Petri dishes filled with regolith, kept in the ScienceDome at room temperature

Control: Petri dishes were placed in the ScienceDome at room temperature without any added stress

After 48h, for the germination study, I isolated spores from the soil samples exposed to the different conditions. Then, to prevent contamination in subsequent steps, I disinfected the spores using various solutions. Once disinfected, I placed four spores on each membrane, which was then folded in half twice. The membranes were then buried in a moistened soil mix within Petri dishes and incubated in the ScienceDome at room temperature.

For the second experiment, I mixed the stressed soils (post-48h exposure) with a soil mix in small pots. After moistening, I transplanted ten plantain seedlings (germinated in the greenhouse approximately one week earlier) into each pot. All pots were labeled and placed in the ScienceDome at room temperature.

On Wednesday 16 April, I will assess spore germination and viability by transferring each membrane to a a separate Petri dish and adding a drop of methyl thiazolyl diphenyl-tetrazolium bromide (MTT) to each spore. Since MTT is photosensitive, I will cover the Petri dishes with aluminium foil and keep them in the ScienceDome at room temperature. After 24h, I will observe the germination and viability of each spore.

On Thursday 17 April, I will evaluate the association between AMF spores and plant roots. All plantain seedlings will be harvested and stained using a series of treatments (bleach, vinegar and ink) at 70°C (oven). After staining, I will observe each seeding roots under a microscope to determine whether there is any point of contact between the AMF and the roots.

During the EVAs, I collected soil samples to identify the AMF species present in the Utah desert soil, depending on their characteristics. If time permits, I will expose the spores found in these samples to the various stress conditions for 48h, then assess their viability with MTT.

Batoul Tani (Crew Journalist):

As part of the microbiological experiments conducted during the MDRS mission, I have been investigating the resistance of two bacterial species, Escherichia coli and Bacillus thuringiensis, to various environmental stressors that simulate Martian surface conditions. These include UV-C radiation, temperature fluctuations, and the potential protective effect of native soil samples.

During the initial phase of the mission, I dedicated my time to the preparation of culture media, including LB broth and agar plates in Petri dishes, to ensure consistent and sterile growth conditions throughout the experiments.

For Escherichia coli, I carried out two main exposure experiments. The first consisted of an 8-hour exposure to UV-C light to evaluate the bacterium’s tolerance to high doses of ultraviolet radiation. The second experiment involved placing the bacterial cultures outdoors for 48 hours, exposing them to natural day-night cycles and ambient temperature fluctuations. These conditions aimed to simulate the thermal variations that would be encountered on the Martian surface. Over the next few days, I intend to monitor the potential development of biofilm structures under these stress conditions, as biofilm formation can serve as a protective survival mechanism in harsh environments.

For Bacillus thuringiensis, I implemented a similar 8-hour UV-C exposure protocol, with an additional variable: the presence or absence of soil collected from the Cowboy Corner site. This was done to explore the potential shielding effect of local soil against radiation. The samples were also subjected to either stable or cyclic temperature conditions to assess how thermal fluctuations interact with UV stress and soil protection. In the upcoming days, I will perform a comparative analysis of colony-forming units (CFUs) to quantify survival rates across the different experimental conditions.

These experiments aim to contribute to our understanding of microbial resilience in analog Martian environments, which has implications for both planetary protection and the feasibility of microbial-based life support systems.

Louis Baltus (Crew Astronomer):

The first experiment I am conducting during the MDRS simulation is centered on developing a solar weather monitoring system using the Musk Observatory. The objective is to design a tool that could one day be deployed on Mars to monitor solar activity and protect astronauts from potentially harmful radiation.

However, the experiment faced delays during the initial phase of the mission. Due to a lack of sufficient prior training and self-education on the telescope’s operation, I was unable to use the observatory effectively for the first several days. This resulted in a significant setback to the planned data collection schedule.

Yesterday, I was finally able to perform my first imaging session with the telescope. This marked a critical step forward, but it also became apparent that the data acquisition process is far more time-consuming than anticipated. Given the remaining time in the mission and the complexity of the equipment, I have decided to focus exclusively on capturing high-quality solar images for the rest of the rotation. The full exploitation and analysis of this data will be carried out after the mission concludes.

Unfortunately, today’s weather conditions were unfavorable, with high winds and heavy cloud cover making telescope operations nearly impossible. Despite this, I remain optimistic. With continued practice and clearer skies in the coming days, I expect my proficiency to improve significantly, allowing for better and more frequent image acquisition.

The second experiment, conducted under the supervision of a professor from UCLouvain, investigates astronaut-computer interaction (ACI) via gesture-based controls—an area that has seen very limited study to date. The goal is to evaluate the practicality and cognitive workload associated with using wearable gesture recognition systems in analog Martian conditions.

Two commercial devices, the TapStrap and TapXR, are being evaluated. Both allow users to input commands via a predefined set of 16 finger gestures. These gestures are intended to serve as an alternative to conventional interaction methods, especially in scenarios where gloves, mobility constraints, or interface limitations would hinder performance.

A baseline data collection session was completed before the mission under standard Earth conditions. During the simulation, I am conducting two test sessions with a participant to measure gesture memorization, recognition accuracy, and production time:

The first session took place on Sol 4, with the participant wearing normal indoor clothing. The session was completed without incident, and all necessary data was recorded.

The second session is scheduled for Sol 8, during which the participant will wear a full astronaut suit. This test will simulate extravehicular activity conditions and allow us to assess how the suit’s physical constraints—such as limited dexterity and sensory feedback—affect gesture-based interaction.

As the experimenter, I am responsible for guiding the participant through the testing protocol and recording all performance metrics. The comparison between the pre-mission, Sol 4, and Sol 8 sessions will provide valuable insights into the feasibility of gesture-based controls for future space missions.

The findings from this study are expected to inform the development of more ergonomic and intuitive human-computer interfaces tailored for constrained environments such as the surface of Mars.

Antoine Dubois (Crew Engineer):

As part of my experiment, I aim to measure sediment transport in an arid environment analogous to that of Mars. The primary objective of this study is to assess wind-driven erosion dynamics and to draw lessons applicable to the protection of structures built on Mars, where extreme climatic conditions and a thin atmosphere suggest a slow but continuous process of erosion.

In the field, I have installed dust collectors at three different heights — 10 cm, 20 cm, and 30 cm — in order to observe variations in particle size distribution depending on their transport height. This approach will help identify whether certain grain sizes are more likely to be transported at specific altitudes, which could have practical implications for the design and durability of Martian infrastructure.

To complement these measurements, I have also deployed a data logger to record local environmental conditions, along with a sensor under each dust collector that measures soil moisture, temperature, and electrical conductivity. These parameters will help me better understand how soil conditions influence sediment movement.

The collected samples will later be analyzed using three different sieves (2 mm, 500 µm, and 250 µm), allowing me to classify the particles by size and create detailed granulometric profiles for each height. These analyses will provide valuable insights into aeolian transport mechanisms in desert environments and their applicability to Martian conditions.

At the midpoint of the mission, all instruments are functioning properly, and the first samples will be collected within a day or two. Initial wind speed and direction data have also been retrieved from the computer available in the HAB. By the end of the mission, this experiment is expected to yield useful and relevant results for research on the adaptation of human infrastructure to the Martian environment.

Béatrice Hollander and Arnaud de Wergifosse (Crew Commander and Crew Executive Officer):

The primary objective of this joint study is to evaluate the effects of a dietary supplementation combining a probiotic (Lactobacillus helveticus) and an amino acid (glycine), compared to a placebo, on stress levels as well as sleep quality and duration. Physiological data have been collected nightly, including heart rate, heart rate variability, total sleep time, and sleep quality, using scientifically validated wearable devices (Oura rings®). Given the highly similar living conditions among crew members during the nighttime period, this timeframe is particularly suitable for such data collection.

To account for potential confounding variables, additional physiological measures such as skin body temperature and oxygen saturation are also monitored. In parallel with these objective physiological indicators, participants completed three self-report questionnaires providing a subjective, behavioral perspective. These instruments assess perceived stress levels (Perceived Stress Scale-10), daytime sleepiness (Epworth Sleepiness Scale), and satisfaction with sleep over the preceding week (PROMIS sleep disturbance).

Furthermore, all crew members engage in a daily session of cardiac coherence—a controlled breathing technique consisting of 5-minute cycles of respiration, characterized by inhaling for 5 seconds and exhaling for 5 seconds. This practice is included to assess its potential effects on stress regulation and sleep, as well as any interaction it may have with the proposed supplementation.

Preliminary trends suggest possible intra-individual variations. Some crew members appear to experience improved sleep duration during the mission compared to their baseline data collected prior to the simulation. However, no significant intergroup differences have been observed at this stage. The majority of the crew has responded positively to the cardiac coherence practice, reporting reductions in stress levels both during and after the sessions.

To date, no significant differences have been identified between the supplementation and placebo groups. In other words, the dietary supplement does not yet appear to exert a notable influence on the measured outcomes.

Astronomy Report – April 13th

[category 

astronomy-report]

Name: Louis Baltus

Crew: 314

Date: 13Apr2025

MUSK OBSERVATORY

Solar Features Observed: Most of the pictures I took this morning were the whole solar disk. Nevertheless, I zoomed in on regions where I saw prominences in order to train myself with the other scales. Sadly, I only had time to finish my first edit, combining two pictures of the same zoom in a prominences region ( see attached ).

Images submitted with this report: (2) The one I’ve just finished editing and one as an example of the problem I encountered with some of my pictures. More pictures will follow as I have a lot of them to edit in the queue. Please feel free to comment on my first final picture so I can make them even better !

Problems Encountered: In the second attached photo, you’ll see an example of the problem I can have with some of my pictures when I try to stack them on the piece of software. Maybe one of you can give me some advice in order to avoid this problem.

End-mission Research Report – April 18th

[category science-report]

Crew 314 – Closing the Airlock: Lessons Learned from Life on the Red Planet

Over the course of two weeks at the Mars Desert Research Station, our crew carried out a wide range of scientific experiments, technical tasks, and field explorations — all within the framework of a simulated Martian mission. From daily EVAs and lab work to emergency drills and group living, each activity helped us better understand the challenges and possibilities of life on Mars.

Through teamwork, adaptability, and shared curiosity, we made progress across diverse fields including medical science, sleep and stress physiology, microbiology, astronomy and soil science. As we prepare to close the airlock for the last time, we leave with new insights, stronger connections, and a lasting sense of what it might mean to live and work on another world.

Experiments:

This section outlines the progress and the final conclusion of the research projects conducted by the crew during our mission.

Odile Hilgers (Health and Safety Officer):

As Health and Safety Officer for this analog Martian mission, I led a complete series of six medical simulations designed to assess Crisis Resource Management (CRM), known as non-technical skills in an isolated and confined environment. These simulations were inspired by realistic emergency scenarios and adapted to the operational constraints of life on Mars.

The training program was carefully structured to increase in complexity and immersion over time. The first two scenarios served as introductory exercises, focusing on familiarizing the crew with medical protocols and simulation dynamics. The following two represented Earth-based emergencies and required a higher level of coordination and critical thinking. The final two simulations introduced Martian-specific constraints, including operational stressors such as limited visibility, environmental alarms, and nighttime execution under sleep-deprived conditions.

These last two Martian scenarios were conducted under particularly challenging circumstances: one at the very end of the night, and the other at the very beginning, both involving full crew wake-up calls, alarm sounds (moderately loud), and operations without electrical lighting—crew members relied solely on their headlamps. These conditions were designed to simulate the kind of fatigue and sensory overload astronauts may face during long-duration missions.

Each simulation followed a structured three-phase format:

Briefing – Participants received clinical background on the scenario, including the patient’s medical history and the narrative of the current illness.

Simulation – Conducted with three role-players and three observers, adapting to realistic crew roles and responsibilities.

Debriefing – A 30 to 45-minute team discussion centered on Crisis Resource Management (CRM) principles. All participants also completed the Ottawa Global Rating Scale (Ottawa GRS) to assess team performance in a structured, reflective manner.

Simulations were held in multiple locations within the station, including both levels of the Hab, the Science Dome and the GreenHab. All six scenarios were successfully completed without incident, and the crew demonstrated adaptability, communication, and coordination throughout the program.

Data collection is now complete. A post-mission analysis will be conducted to examine behavioral patterns, decision-making processes, and overall team dynamics under pressure. This evaluation aims to contribute to the broader understanding of medical team performance in analog space missions and inform the design of future training protocols for long-duration spaceflight.

Bérengère Bastogne (GreenHab Officer):

The experiments conducted at the Mars Desert Research Station are part of my doctoral thesis. The main objective is to evaluate the impact of Martian environmental stresses – UV radiation (A, B and C), temperature (hot-cold cycles), gravity and substrate (regolith) – on arbuscular mycorrhizal fungi (AMF). These fungi are obligate symbionts that associate with plant roots and can supply them up to 80% of total phosphorus and nitrogen. As one of the most important mutualistic microorganisms for global food production, AMF are essential elements to be considered for the development of future colonies on Mars.

Understanding how AMF responds to environmental conditions is critical. Since they are closely associated with plants, any parameter affecting spores could impact essential AMF functions and indirectly impact plant growth and plant health. However, despite their crucial role, little is known about how these environmental stresses affect these microorganisms. Therefore, expanding our knowledge in this area is crucial.

My research is divided into two experiments. The first aims to study the effects of these stresses on spore germination. The second focuses on the ability of spores, after exposure to stresses, to associate with plant roots.

The initial step involved estimating the number of spores in 10 g soil (to prepare for the first and second experiments). I then exposed the soil – containing spores – in Petri dishes or Falcon tubes to different conditions for 48h. For the germination study, approximately 10 g of soil (containing 30-40 spores) was used per condition. For the root association study, I used 10 g per condition, with six replicates.

To test the substrate stress, I isolated spores from 6x10g of soil and transferred them into regolith. The environmental conditions were applied as follows:

Temperature: Petri dishes were placed outside (near the entrance of the ScienceDome)

Gravity: Falcon tubes were attached to the Random Positioning Machine (RPM) – placed in the ScienceDome at room temperature

UV: Petri dishes were placed under a UV lamp in the ScienceDome at room temperature

Substrate: Spores were placed in Petri dishes filled with regolith, kept in the ScienceDome at room temperature

Control: Petri dishes were placed in the ScienceDome at room temperature without any added stress

After 48h, for the germination study, I isolated spores from the soil samples exposed to the different conditions. Then, to prevent contamination in subsequent steps, I disinfected the spores using various solutions. Once disinfected, I placed four spores on each membrane, which was then folded in half twice. The membranes were then buried in a moistened soil mix within Petri dishes and incubated in the ScienceDome at room temperature.

For the second experiment, I mixed the stressed soils (post-48h exposure) with a soil mix in small pots. After moistening, I transplanted ten plantain seedlings (germinated in the greenhouse approximately one week earlier) into each pot. All pots were labeled and placed in the ScienceDome at room temperature.

On Wednesday 16 April, I assessed spore germination and viability by transferring each membrane to a separate Petri dish and adding a drop of methyl thiazolyl diphenyl-tetrazolium bromide (MTT) to each spore. Since MTT is photosensitive, I covered the Petri dishes with aluminium foil and kept them in the ScienceDome at room temperature. After 24h (Thursday 17 April), I observed the germination and viability of each spore under microscope.

On Thursday and Friday, 17 and 18 April, I evaluated the association between AMF spores and plant roots. All plantain seedlings were harvested and stained using a series of treatments (bleach, vinegar, and ink) at 70°C (oven). After staining, I observed each seeding root under a microscope to determine whether there was any point of contact between the AMF and the roots.

During the EVAs, I collected soil samples to identify the AMF species present in the Utah desert soil, depending on their characteristics. I observed the presence of mycelium and spores in some soils.

I placed the soil samples in Petri dishes and inoculated them with spores of arbuscular mycorrhizal fungi. The dishes were kept at room temperature for 48h. After this, I collected the spores, transferred them onto a membrane and added a drop of MTT solution. After 24h, I examined each spore under a microscope to assess its viability.

In addition, I subjected soil samples collected during the EVAs to different stress conditions (temperature, UV, gravity and control). Once back in Belgium, I will analyze the viability of the spores from these soils following the stress treatments.

Batoul Tani (Crew Journalist):

As part of the microbiological experiments conducted during the MDRS (Mars Desert Research Station) mission, I investigated the resistance of two bacterial species, Escherichia coli and Bacillus thuringiensis, to various environmental stressors simulating Martian surface conditions. These stressors included UV-C radiation, temperature fluctuations, and the potential protective effect of native soil samples.

During the initial phase of the mission, I focused on preparing culture media, including LB broth and agar plates in Petri dishes, to ensure sterile and consistent growth conditions for the duration of the experiments.

For Escherichia coli, I conducted two main exposure experiments. The first involved an 8-hour exposure to UV-C light to assess the bacterium’s tolerance to high doses of ultraviolet radiation. The second experiment consisted of a 48-hour outdoor exposure, subjecting the cultures to natural day-night cycles and ambient temperature variations—conditions designed to mimic thermal changes on the Martian surface. In the coming days, I plan to monitor the potential development of biofilm structures under these stress conditions, as biofilm formation can serve as a protective survival mechanism in extreme environments. Optical density measurements will be carried out in Belgium to quantify the differences between the experimental groups.

For Bacillus thuringiensis, I applied a similar 8-hour UV-C exposure protocol, introducing an additional variable: the presence or absence of soil collected from the Cowboy Corner site. This allowed for the exploration of a potential shielding effect provided by the local soil against UV radiation. The samples were also exposed to either stable or cyclic temperature conditions to investigate the interaction between thermal stress, UV exposure, and soil protection.

I analyzed the differences in colony-forming unit (CFU) counts across the various conditions, and some significant trends have begun to emerge. However, I would have liked to examine whether the bacteria underwent sporulation, as this is a key survival strategy for Bacillus thuringiensis. Unfortunately, the microscope available on site was not of sufficient quality to allow for such observations.

These experiments aim to contribute to our understanding of microbial resilience in Martian analog environments, with implications for planetary protection protocols and the development of microbial-based life support systems for future space missions.

Louis Baltus (Crew Astronomer):

The first experiment I conducted during the MDRS simulation aimed at developing a solar weather monitoring system through the use of the Musk Observatory. The objective was to establish a protocol for solar imaging and data collection that could eventually support forecasting tools to protect future astronauts from solar radiation exposure on Mars.

The early stages of the mission were hindered by a delay in my personal training on the telescope’s operations. As a result, I lost valuable observation days during the first part of the rotation. Despite this initial setback, I successfully conducted my first imaging session mid-mission, and continued making observations whenever weather conditions permitted.

Unfortunately, after Sol 6, the weather became increasingly uncooperative. Strong winds and dense cloud cover significantly reduced available observation windows. I nonetheless maintained efforts to operate the telescope during every favorable interval. By the end of the simulation, I had accumulated a modest but valuable collection of solar images.

Once I return to Belgium, I will carry out a detailed post-processing and analysis phase. The goal will be to assess whether the quantity and quality of the captured data are sufficient to move forward with the development of a basic solar weather monitoring prototype.

Regardless of the limited observation time, this experience has been extremely rewarding on a personal and scientific level. Learning to operate a solar observatory and observing the Sun with my own eyes has been a profound and unforgettable discovery. I remain deeply grateful to all those who made this opportunity possible.

The second experiment, supervised by a professor from UCLouvain, investigated gesture-based astronaut-computer interaction (ACI) — a field with very limited prior research. The aim was to evaluate the cognitive and physical feasibility of using wearable gesture-recognition devices in analog Martian conditions. The two systems tested were the TapStrap and the TapXR.

The experimental protocol was composed of four distinct measurement sessions:

A baseline session, conducted pre-mission under standard terrestrial conditions.

A first in-mission session on Sol 4, performed with the participant in regular indoor clothing.

A second in-mission session on Sol 8, carried out under extravehicular activity (EVA) conditions, with the participant wearing a full one-piece astronaut suit.

A final post-mission session, scheduled to take place in Belgium, which will complete the full dataset.

The Sol 8 session provided particularly valuable data, as it tested gesture input performance under conditions of reduced dexterity and tactile feedback. Despite the additional constraints imposed by the EVA suit, the session was executed smoothly, and all required data was successfully collected.

As the experimenter, I oversaw each session, guided the participant through the sequence of 16 predefined gestures, and systematically recorded performance metrics including memorization accuracy, recognition rate, and execution time. This structured approach will allow for a comprehensive comparison across different environmental and ergonomic conditions.

Upon completion of the final post-mission session, I will work in collaboration with my supervising professor to conduct a full analysis of the results. The aim is to draw conclusions regarding the viability and limitations of gesture-based interfaces in space mission scenarios.

This research offers promising perspectives for the development of alternative control systems for future planetary missions—especially in environments where traditional input methods are limited or impractical.

Antoine Dubois (Crew Engineer):

As part of my mission at the Mars Desert Research Station (MDRS), I conducted an experiment aimed at better understanding wind-driven erosion dynamics in an arid environment analogous to Mars. The goal was to assess erosion caused by wind and draw lessons to help protect infrastructure under extreme Martian conditions, where the thin atmosphere and harsh climate suggest a slow but persistent erosion process.

In the field, I installed three dust collectors at different heights (10 cm, 20 cm, and 30 cm) to observe variations in particle size distribution depending on transport height. This aimed to determine whether specific grain sizes are more likely to be transported at certain altitudes, which could inform the design and durability of Martian structures.

I also deployed a data logger connected to three sensors placed under each collector to measure soil moisture, temperature, and electrical conductivity, helping to understand how soil conditions influence sediment mobility.

Initially, all devices worked correctly, and the first samples were collected shortly after mid-mission. However, due to poor weather and difficult terrain access, the equipment was retrieved on SOL 11 instead of SOL 12 as planned.

A technical issue occurred: although the sensors operated correctly, a configuration error in the data logger prevented environmental data from being recorded, limiting direct analysis of on-site measurements.

Despite this, I retrieved complementary environmental data from the station’s iMac, including wind speed and direction, ambient humidity, and rainfall events — all valuable for interpreting the experiment’s results.

The collected dust samples were sieved using three mesh sizes (2 mm, 500 µm, and 250 µm) and weighed on site. Detailed particle size analyses will be performed after returning to Earth (Belgium) to produce precise granulometric profiles.

Despite the challenges, the experiment yielded usable samples and coherent environmental data for interpretation. This contributes to a better understanding of aeolian transport in deserts and may support the design of infrastructure suited to Martian conditions.

Béatrice Hollander and Arnaud de Wergifosse (Crew Commander and Crew Executive Officer):

As part of an investigation into human resilience in isolated and confined environments, this study evaluated the effects of a dietary supplement combining Lactobacillus helveticus and glycine, compared to a placebo, on stress regulation and sleep during a two-week analog mission at the Mars Desert Research Station (MDRS). Crew members were monitored nightly using wearable devices (Oura rings®) that tracked physiological indicators such as heart rate, heart rate variability, total sleep time, and sleep stages. Self-reported stress, sleep satisfaction, and daytime sleepiness were also assessed through validated questionnaires.

All participants practiced daily cardiac coherence breathing, a controlled technique designed to promote autonomic regulation. While no significant differences were found between the supplement and placebo groups, some participants reported consistently positive effects from cardiac coherence, including reduced stress and improved emotional regulation.

Interestingly, sleep became more fragmented during the second week, coinciding with nighttime emergency simulations. Despite these disturbances, perceived stress levels paradoxically declined, possibly reflecting psychological adaptation to the mission environment or due to the breathing technique. Furthermore, some participants also exhibited clinically relevant daytime sleepiness.

Although the supplementation showed no clear effect over the short term, the breathing practice proved to be a well-received and low-effort countermeasure. Full statistical analyses will follow to better interpret the collected data and clarify emerging trends.

Crew 309 End-Mission Research Report 22Mar2025

[title End-Mission Research Report – March 22nd]

[category science-report]

Crew 309 Mission Science Report
MDRS, Saturday 22 March 2025

Name of person filing report: Aaron Allred
Overview: Crew 309 has been in the MDRS for one week, conducting a pilot
experiment within the research domain of aerospace human factors research. Due to
the nature of these experiments, the six-person habitat crew has remained naïve to
the experiment objectives, which have been coordinated by a separate three-person
experimental team stationed in nearby Hanksville, UT while running remote
operations by day out of the science dome.
In particular, our primary experiment examined how aspects of teams, confined to an
isolated space analog, influence information processing, communication, and
performance amongst dyads performing simulated EVAs. As such, our experiment
required different combinations of the six-person habitat crew to perform a series of
12 EVAs over the course of the week. During these EVAs, the crew was in full sim.
Due to the ongoing nature of this experiment (and being a pilot test), some of the
finer experimental details are left intentionally vague. Please feel free to contact us.
Name: Extravehicular Activity Navigation Tasks
Type: Human Factors: Judgments and Decision-Making
Crew: Full Crew
Description: The crew performed a series of 12 EVAs over the course of a week
(each lasting ~3 hours) in various dyad pairs. These pairs were structured by the
experimenters a priori to produce unique combinations across team members, who
were each assigned unique roles including Co-Commander A, Co-Commander B,
Scientist, Engineer, Medic, and GreenHab. These roles provided an assigned
hierarchy that was assessed for natural evolution throughout the mission week using
subjectively reported nightly surveys, as well as after EVAs.
During these EVAs, which represent unique experimental trials, crewmembers were
assigned unique pieces of information that could not be shared physically but could
be shared using other forms of communication (such as over radio). This portion of
the design used distributed information sources to simulate distributed expertise that
may be encountered during future space missions EVAs.
Navigation performance was gathered using Garmin T5X GPS tracking equipment in
real-time. Experimenters had access to a Garmin Alpha 10 handheld and Drivetrack
71, which enabled this real-time tracking and monitoring. In addition to voice and
position sensors, pilot participants were outfitted with physiological sensors, which
included eye-tracking glasses. For this pilot experiment the Tobii 2 was utilized, but in
future experiments, the Tobii 3 is planned. Following EVAs and at night,
crewmembers filled a number of surveys. To facilitate these devices, custom
spacesuit simulators were designed and engineered for this mission.

A vehicle in a desert AI-generated content may be incorrect.

Figure 1 . Return from a dyadic navigation task on the final day.

Status:
All experimental conditions were successfully piloted by the crew over the course of
the course of the simulation. In addition to executing these tasks in dyads,
crewmembers also successfully made autonomous repairs to their custom spacesuit
simulators, eye-tracking equipment, and survey software. This pilot experiment
demonstrated that a trained crew is capable of performing autonomous, in-simulation
human factors research at MDRS for full data collection at a later date. Further, this
pilot experiment gave key insights into how to titrate the task difficulty at each of our 6
EVA sites, streamline experimental operations, and provide a more representative
spaceflight-analog isolation and confinement experience to crew members.

Astronomy Report – February 17th

[category 

astronomy-report]

Astronomy Report
Name: Quentin Berlemont
Crew: 311
Date: 02/17/2025

MDRS ROBOTIC OBSERVATORY
Robotic Telescope Used (choose one) MDRS-14 MDRS-WF RCOS-16
Objects to be Imaged this Evening:
Images submitted with this report:
Problems Encountered:
MUSK OBSERVATORY
Solar Features Observed: few sunspots + protuberances
Images submitted with this report: 2
Problems Encountered: I followed all the steps for sun imaging, it
renders great until I select "Stack" on sharpcap, which makes a double
of the original image and place it on top, as you can see on the first
image linked. It is not related to a long exposure time as I only took
1000 images for about 3 seconds. Could you help me with this issue? Also
I see absolutely no difference both in the eyepiece and the camera when
turning either tuners, maybe they are too far engaged? What should I do?

Astronomy Report – February 18th

[category 

astronomy-report]

Crew 311 Astronomy Report 18Feb2025
Name: Quentin Berlemont
Crew: 311
Date: 02/18/2025
MDRS ROBOTIC OBSERVATORY
Robotic Telescope Used (choose one) MDRS-14 MDRS-WF RCOS-16
Objects to be Imaged this Evening:
Images submitted with this report:
Problems Encountered:
MUSK OBSERVATORY
Solar Features Observed: few sunspots
Images submitted with this report: 2
Problems Encountered: I did as you told for the tuners, it seems to
work! On my previous report I confused the program names, I meant "stack
on registax". It seems that the issue was due to the telescope moving
somehow because today the pictures are clear! Do you have advices on how
to set the tuners to make the best images of the chromosphere or
prominences?

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